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Questions related from Chris Noakes
I want to collect blood samples (as much volume as possible) from cardiac puncture. Does anyone have a good protocol for how to collect and process the samples? For example what is the...
11 November 2013 575 17 View
We want to see if our mice lines have any difference in blood biochemistry and/or haematology. We were planning on taking blood from anaesthetised mice via the cardiac puncture method prior to...
10 October 2013 7,158 18 View
I have created several dox inducibles (tetON) transgenic mouse lines from injection of DNA into embryos and random integration into the genome. These lines have all been genotyped for the presence...
10 October 2013 9,225 7 View
I am trying to fix and stain mammospheres formed on matrigel in chamber slides using MCF10A cells. I am having difficulty retaining the formed mammospheres on the matrigel. All steps have been...
01 January 2013 7,231 15 View
I am trying to ligate an insert into a vector using 5' EcoN1 and 3' EcoR1 sites. These are realistically the only sites I can use to get the insert in. After cutting with EcoN1 I am left with a...
11 November 2012 1,006 11 View
I'm trying to purify 2-5 ug of my vector insert from a plasmid digest for injection into mouse embryos to make transgenic mice. I'm digesting 10-20 ug of maxi prepped vector and running it on a...
11 November 2012 8,145 14 View
I'm having real difficulty in generating a stable MCF10A cell line that expresses the TetR in Clontech's Tet-ON system. Currently, I am electroporating ~1.5 x106 cells with 10 ug of linear (ScaI...
05 May 2012 5,245 18 View
