Chris Halkides

2 Questions 5 Answers 0 Followers

Questions related from Chris Halkides

How does one calculate the best volume of nickel gel to use in a protein purification?

We know the capacity of the gel and the total amount of protein in the bacterial lysate from a Bradford assay. What we do not know is what fraction of the total protein is the his-tagged protein...

16 November 2020 4,785 2 View

Are there any tricks to making up one's own Bradford reagent for protein assays?

We think it would be cheaper to make our own from the solid dye, phosphoric acid, and an alcohol. I think that using acid-washed glassware makes sense, to minimize interference from detergents....

01 January 1970 8,826 4 View