Choi Wonseok

2 Questions 4 Answers 0 Followers

Questions related from Choi Wonseok

Why yeasts do not grow after transformation?

Hello, I have trouble with dealing with yeasts. My problem is essentially 'yeasts are not growing'. The yeast is S.cerevisiae, VL6-48 strain. I bought this strain from ATCC, and growing well in...

17 August 2021 5,027 6 View

Reason for protein aggregation during incubation with Ni-NTA beads?

Hello, I am facing a problem related to protein aggregation. I am currently trying to solubilize and refold a his-tagged kinase from inclusion bodies. There are no precipitates in the refolded...

10 June 2020 5,291 6 View