Chitrali Laha Roy

2 Questions 5 Answers 0 Followers

Questions related from Chitrali Laha Roy

Ligation using T4 DNA Ligase?

I am currently performing ligation of my Bcl1 digested product and for one reaction I have to add 5U of T4 DNA Ligase per 250uL of total reaction volume. The enzyme I recieved, had explained its...

21 August 2024 4,017 4 View

Intron -22 Inversion for FVIII gene?

Currently, I am trying to perform the Intron 22 Inversion for the FVIII gene. I have tried both long-distance (LD) PCR and Inverse Shift (IS) PCR, but I was unable to get the results in either...

29 July 2024 3,258 4 View