Chitra Nair

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Questions related from Chitra Nair

How to remove strongly bound proteins from a size exclusion column?

I use biosep SEC s2000 column which has a column volume of 15 ml a peak is observed at 47 min in all runs, even when I pass BSA through the column. I have tried washing the column with 0.1 M...

04 July 2019 7,691 5 View

Why do proteins stack at the top of the gel in zymography by co-polymerization of substrate with gel?

I am trying to ascertain the Fibrinolytic activity of 3 of my proteins by zymography. One of the proteins is a low Molecular weight protein while the other two are about 25-30kDa in size. However,...

22 November 2018 725 3 View