Chichi Okagbue

3 Questions 7 Answers 0 Followers

Questions related from Chichi Okagbue

Lysotracker staining on frozen tissue sections on slides?

I would like to stain the lysosomes of adipose tissue sections I have already collected on slides. I am aware that Lysotracker probes are primarily for live cells however I was directed by PI that...

12 June 2024 9,488 0 View

Issues with uneven loading probing with loading controls?

I am currently doing Western Blots for adipose tissue lysates however I am having a hard time getting even loading especially when probing for loading controls (B-Actin and A-Tubulin). I feel like...

31 January 2023 6,987 0 View

Western Blot Stripping on Nitrocellulose Membranes No Heat Alternative?

I am planning to strip my current membrane in order to test a new antibody however I have some obstacles in regards to my protocol. My stripping buffer recipe and protocol is as follows:- 1M Tris...

08 December 2022 5,114 13 View