Cheuk Tam

5 Questions 9 Answers 0 Followers

Questions related from Cheuk Tam

Why are the bands so irregular in this western blot?

Hi, I'm wondering why the first blot (_170858) shows such wavy bands. Is it an issue with the gel or the transfer? Also the lower blot in the second image (_172622) shows a decreasing signal in...

16 April 2016 6,048 3 View

What are the principles behind subtracting/normalizing background fluorescence?

One manufacturer protocol suggests subtracting background fluorescence from the raw fluorescence reads (F - Fo). Other protocols suggest dividing F/Fo. Yet another protocol suggests taking (F -...

12 February 2016 5,825 8 View

What are good criteria for neurite outgrowth and neuron migration from a neurosphere?

Hi, I'm trying to find reproducible and efficient criteria for quantifying neurite outgrowth and neuron migration from human-derived neurospheres. My lab has suggested references in the attached...

30 January 2016 1,963 0 View

How can I troubleshoot an issue with FIJI's Stitching plugin's misalignment of mosaic images?

Hi All, I was using the plugin for 10X images of neurospheres and ran into issues with alignment. I took images in a snake pattern with a lot of overlap (>50%). I then used the "unknown...

17 October 2015 5,905 4 View

What are good max self complementarity and max 3' self complementarity scores to use as cutoffs when designing qPCR primers with Primer3?

Are the default values of 8 and 3, respectively, stringent enough to avoid primer dimers and hairpins? Thank you so much.

08 November 2014 4,413 3 View