Chetan Arya

3 Questions 8 Answers 0 Followers

Questions related from Chetan Arya

Expression of multi disulfide bonds containing protein in bacterial system?

I have a insect protein which contains 4-6 disulfide bonds in its active form. I want to use a bacterial expression system (or yeast if it does not work out for bacteria) for over-expression of a...

06 June 2019 8,014 6 View

Comparing activity of an enzyme with different substrates?

My question refers to the fundamental problem of enzyme kinetics. I am working on a hydrolase which is specific for (lets say) substrate "A". I perform a real time kinetic assay to determine the...

11 November 2018 6,375 5 View

What information/s can I get from the data in which I excited my protein at 383nm and got emission at 450nm. ?

My protein has color of brown but in UV_Vis spectroscopy no distinct band after 280nm. At high conc. of protein I can observe at shoulder at 383nm so I excited my protein sample at 383nm and took...

12 December 2014 694 11 View