Chet Ram

4 Questions 22 Answers 0 Followers

Questions related from Chet Ram

Whether PCR product generated by degenerate primers can be sequenced directly?

I have designed degenerate primer pairs from heterologous systems and amplified in genomically less-explored plant species. Since degenerate primers amplify all possible related sequences [gene...

10 October 2018 7,115 3 View

Position of alternative spliced variants on the chrmosomes?

I have mined some gene families from a genome. I am getting some genes with high sequence similarities however they are residing at the different position on the chromosome. So my query is that...

03 March 2017 3,525 4 View

How can I calculate the gene expression based on RPKM method?

Runs: 1 run, 23.6M spots, 4.7G bases, 2.8GbRun # of Spots # of Bases Size SRR3156560 23,576,012 4.7G 2.8Gb From the above mentioned data...

11 November 2016 5,599 0 View

Is there any study of RNA in situ hybridization for biotic stress in plants?

I am doing aphid induced gene expression analysis in Brassica so I would like to know how aphid is expressed genes for its own survive, whether it suppressing plants defense mechanism or it...

02 February 2014 5,079 3 View