Why my dh5a always flocculated when cultured in M9 medium?

I used the M9 supplemented medium but didn't adjust the pH of 5x M9 salts to 7.2. The final concentration of Ampicillin is 100ng/mL. Here is my recipe: 1X M9 salts 1mM thiamine...

31 October 2020 2,670 1 View