Chen Lesnik

5 Questions 9 Answers 0 Followers

Questions related from Chen Lesnik

Imaging- permeabilization without fixation?

Hi, Has anyone ever tried to permeabilize cells (or isolated organelles) without fixation first? Thanks!

04 March 2021 3,093 3 View

Seahorse- isolated mitochondria- lower OCR after adding FCCP?

Hi, When studying the respiratory function of isolated mito. by measuring OCR using the Seahorse- I'm using a buffer (MAS) that contains pyruvate+ malate. After measuring the basal respiration-...

06 August 2019 7,723 6 View

Mass spec. analysis- emPAI vs. NSAF?

Which method do you find better to compare between 2 samples? what are the thing I should take into account when using either of them (normalization etc.)?

17 July 2019 3,597 2 View

How to analyze unique proteins when comparing 2 datasets of proteomic analysis?

Hi, I'd like to compare between mass spec. results of 2 samples that I have (each sample has 3 biological replicates). Following several filtration steps- I have a list of proteins that were...

17 July 2019 7,061 8 View

Using Seahorse to measure respiration- both pyruvate+ malate are needed?

I saw that in most protocols- when measuring respiration (of isolated mitochondria, using the Seahorse), both pyruvate+ malate are added. I was wondering what would be the result of adding only...

09 February 2019 6,738 5 View