Che-Min Lo

4 Questions 5 Answers 0 Followers

Questions related from Che-Min Lo

Any suggestion on the problem of nuclear membrane lysis and false-positive of IgG negative control in Chromatin immunoprecipitation (ChIP assay ) ?

Dear all I would like to ask some questions about my current ChIP experiment. I bought the ChIP assay kit from Cell Signaling Technology (#9002 SimpleChIP @ Enzymatic Chromatin IP kit with...

19 March 2015 9,495 3 View

Using 5' phosphorylated primers to perform inverse PCR for getting deletion construct.

I want to do the inverse PCR using 5’ phosphorylated primers to get my interested deletion constructs by deleting (skipping) the internal region. I have already phosphorylated the primers with T4...

29 March 2013 8,654 1 View

The usage of MatInspector promoter analysis tool

My current research is about a gene transcription regulation. I try to find its promoter region and the putative transcription factor-binding elements. After I found out the upstream of 5'...

15 October 2012 7,254 4 View

[ Help ] The failed ligation of my DNA vector and PCR insert

I need some help ~ I have done the ligation Cloning many times, but there was always disappointed. The major problem is that I don't know why the failed ligation of vector and PCR insert. The...

04 October 2012 3,028 5 View