Chase Hellmer

3 Questions 2 Answers 0 Followers

Questions related from Chase Hellmer

Does anyone have experience using the Chat-Cre mice lines from Jackson? I am having trouble getting the PCR bands to show up during genotyping?

'GCA AAG AGA CCT CAT CTG TGG A' - WT Forward Primer 'CAG GGT TAG TAG GGG CTG AC' - Common Primer 'CAA AAG CGC TCT GAA GTT CCT' - Mt Forward Primer PCR Protocol is attached. In brief, used as low...

03 March 2019 6,942 6 View

Can excess bicarbonate in Ames solution impair light response experiments in the mouse retina?

I typically add bicarbonate until the pH of the solution is 7.4, although sometimes it is slightly above.

01 January 2017 4,831 0 View

At what concentration of intracellular calcium does apoptosis/necrosis begin to occur? Higher than 1mM?

For patch clamp experiments involving excitotoxicity, how much pipette calcium should be used to be equivalent to an exitotoxic level? Higher than 1mM? I know the typical level is around 100nM but...

08 August 2016 8,020 2 View