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Questions related from Chase Hellmer
'GCA AAG AGA CCT CAT CTG TGG A' - WT Forward Primer 'CAG GGT TAG TAG GGG CTG AC' - Common Primer 'CAA AAG CGC TCT GAA GTT CCT' - Mt Forward Primer PCR Protocol is attached. In brief, used as low...
03 March 2019 6,957 6 View
I typically add bicarbonate until the pH of the solution is 7.4, although sometimes it is slightly above.
01 January 2017 4,848 0 View
For patch clamp experiments involving excitotoxicity, how much pipette calcium should be used to be equivalent to an exitotoxic level? Higher than 1mM? I know the typical level is around 100nM but...
08 August 2016 8,038 2 View