Charles Zogzas

4 Questions 2 Answers 0 Followers

Questions related from Charles Zogzas

Has anyone experienced any metal binding properties of GST fusion protein?

I am using a GST-fusion construct for a metal-binding protein that we work on. We avoid a traditional 6x-His tag because Histidine residues have a fairly strong affinity for metal ions (hence, why...

19 October 2015 7,161 2 View

What is the most crude solution that an FPLC column (gel filtration) can manage?

I am considering assaying oligomerization of my membrane protein. I am wondering if it is possible to run a clarified mammalian cell lysate over a gel filtration column using FPLC. We have the...

05 October 2015 6,935 7 View

Can you skip the recovery portion of transformation if you are using ampicillin resistance?

dh5a cells is what I am typically using and the 30-45 minute outgrowth or recovery period is a nuisance and I have heard that it is not always necessary depending on the mode of action of your...

31 July 2015 3,174 3 View

Does Glycine in solution go bad? I use it for Block B on immunofluorescent staining and wondered if Glycine has a shelf life?

Usually lasts about a month before I smell that something has gone bad. I believe it is likely the FBS that spoils but wondered if old glycine could also cause the issue. Thanks

15 June 2015 4,367 1 View