Chanqqing Yuan

3 Questions 4 Answers 0 Followers

Questions related from Chanqqing Yuan

Hyperphage, M13KO7ΔpIII

In the paper below, "To obtain hyperphage, M13KO7ΔpIII DNA was electrotransfected into E. coli DH5 /pIII." Why not to use hyperphage M13KO7ΔpIII to transfect E. coli DH5 /pIII? Thank you very...

08 March 2016 9,935 2 View

Hyperphage, M13KO7ΔpIII

In the paper below, "To obtain hyperphage, M13KO7ΔpIII DNA was electrotransfected into E. coli DH5 /pIII." Why not to use hyperphage M13KO7ΔpIII to transfect E. coli DH5 /pIII? Thank you very...

03 March 2016 9,749 2 View

I want to insert a DNA fragment (not a plasmid) containing Ampicillin resistance into TG1 genome to get stable cell lines. I have 2 questions?

1. How to digest plasmid completely, without even one copy of intact circular plasmid? 2. Is it possible to insert this DNA fragment into the genome of TG1 cells randomly? It looks possible from...

02 February 2016 8,405 3 View