Chanel Blignaut

4 Questions 2 Answers 0 Followers

Questions related from Chanel Blignaut

SDS PAGE: Gel running very slow?

I made a 12.5% glycine gel for protein analysis. Materials: 12.5% separating buffer (1.5M Tris pH 8.8) 4% stacking buffer (0.5M tris pH 6.8) Monomer solution (30% acrylamide + 2.7% bis...

28 August 2023 8,483 3 View

Analyzing Absorbance Spectrum of CBD: How do I fix my graph?

I measured the absorbance of CBD (1mg/ml in methanol) diluted in HEPES buffer (0.2M NaCl and 20mM Hepes pH 7.5). I have attached a picture of my results - however it does not resemble what is...

22 July 2023 7,029 2 View

I am doing time resolved FTIR between a protein and CBD. My protein is in HEPES buffer - but the spectrum only picks up the buffer and not my protein?

I am doing time resolved FTIR between a protein and CBD. My protein is at 66uM and the CBD at 1250uM - both in Hepes buffer (20mM hepes, 200mM NaCl pH 7). I tried a lower concentration of CBD in...

30 June 2023 3,582 1 View

Is transcription factor dimerization physiologically relevant

Some transcription factors can dimerize and interact with DNA. However, is this physiologically relevant considering the nucleus is under reducing conditions and therefore the transcription...

01 January 1970 3,221 1 View