Chandrajeet Singh

3 Questions 4 Answers 0 Followers

Questions related from Chandrajeet Singh

How have the names of potyvirus proteins been derived?

some of the proteins of potyvirus like HC-Pro, NIa, CP are from Host component, Nuclear inclusion, and coat protein respectively but what are the meaning of P1, P3, 6K1, and 6K2 means how these...

18 May 2015 6,400 3 View

Hello dear..has anyone an idea about blunt end cloning by phusion green hot start dna polymerase?

Actually i m trying to amplify 9.7kb insert by phusion green hot start dna polymerase but I don't get amplified product at all. I changed some parameter of pcr cycle and reaction mixture but still...

02 February 2015 7,832 2 View

How can 5` RACE and 3` RACE help to amplify terminal portion of potyvirus?

For 3` terminal region of potyvirus we can use poly A tail but for 5`the terminal internal sequence is required. Before sequencing we can`t know about the 5` terminal, so my question is, how can...

07 January 2015 7,385 1 View