Chandrajeet Singh

4 Questions 5 Answers 0 Followers

Questions related from Chandrajeet Singh

How have the names of potyvirus proteins been derived?

some of the proteins of potyvirus like HC-Pro, NIa, CP are from Host component, Nuclear inclusion, and coat protein respectively but what are the meaning of P1, P3, 6K1, and 6K2 means how these...

18 May 2015 6,417 3 View

Hello dear..has anyone an idea about blunt end cloning by phusion green hot start dna polymerase?

Actually i m trying to amplify 9.7kb insert by phusion green hot start dna polymerase but I don't get amplified product at all. I changed some parameter of pcr cycle and reaction mixture but still...

02 February 2015 7,851 2 View

Can anyone let me know how much of an insert size (? kb) can be cloned in TA cloning vector?

Hello friends, I'm trying to clone cDNA amplified product of 9.7kb in TA cloning vector but i could not cloned it. anyone let me know how much length insertion size of TA cloning vector? if TA is...

19 January 2015 4,779 5 View

How can 5` RACE and 3` RACE help to amplify terminal portion of potyvirus?

For 3` terminal region of potyvirus we can use poly A tail but for 5`the terminal internal sequence is required. Before sequencing we can`t know about the 5` terminal, so my question is, how can...

07 January 2015 7,406 1 View