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I have isolated total RNA from mammalian cell culture samples. I used a Qiagen Kit. I have checked the conc and quality of RNA by Nanodrop. Ratio of 260/280 was between 2.01 and 2.09, and 260/230...
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I am measuring a gene which is involved in differentiation of a cell. During differentiation its level is high, compared to undifferentiated state. Upon terminal differentiation of cell, mRNA...
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