Chaitanya Dingare

5 Questions 4 Answers 0 Followers

Questions related from Chaitanya Dingare

Mortality in fish tanks?

Hi all, Recently, we have experienced a heavy mortality in our fish tanks. More specifically, we observed 6-7 dead fish in a single tank per day. It's reduced now to 1-2 fish a tank per day but...

07 May 2018 8,169 6 View

Immuno-staining for a nuclear protein after WISH on an adult zebrafish sample

Dear All, I wish to perform WISH on an adult zebrafish sample which is treated with collagenase and later acid-fixed (Formaldehyde + Acetic Acid). But before the WISH procedure, I would like to...

14 June 2017 6,014 1 View

Is there any way to perform whole mount ISH on adult zebrafish tissue such as ovary?

Dear Researchers, I want to perform a whole mount ISH experiment on the zebrafish ovary. There are many papers in which they have done this experiment but the details are kind of missing. What I...

09 June 2016 6,400 3 View

GFP Knock-in by TALEN in zebrafish?

Dear All, I have been trying to knock-in GFP in a particular locus at its N-terminus. I have prepared the Donor construct with a short arm of 0.8 kb and a long arm of 1.6 kb. I have been doing...

24 November 2015 8,999 4 View

Is there anyone with low midiprep yield?

Hi all, I am trying to make a construct by Gibson Cloning (NEB Kit). I have assembled 4 fragments and the total size is 6.5 kb. These fragments contain some intronic sequences from zebrafish and...

20 June 2015 3,640 3 View