3 Questions 2 Answers 0 Followers
Questions related from Chaitanya Dingare
Dear All, I wish to perform WISH on an adult zebrafish sample which is treated with collagenase and later acid-fixed (Formaldehyde + Acetic Acid). But before the WISH procedure, I would like to...
14 June 2017 6,002 1 View
Dear All, I have been trying to knock-in GFP in a particular locus at its N-terminus. I have prepared the Donor construct with a short arm of 0.8 kb and a long arm of 1.6 kb. I have been doing...
24 November 2015 8,997 4 View
Hi all, I am trying to make a construct by Gibson Cloning (NEB Kit). I have assembled 4 fragments and the total size is 6.5 kb. These fragments contain some intronic sequences from zebrafish and...
20 June 2015 3,636 3 View