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Questions related from Chaitanya Dingare
Hello, I am trying to find a minimal length of homology arms required to knock in small tags in the mouse ES cells. My tag is 207 bp long and wondering I need to have long homology arms. Plus, I...
29 January 2021 4,967 0 View
Hi all, Recently, we have experienced a heavy mortality in our fish tanks. More specifically, we observed 6-7 dead fish in a single tank per day. It's reduced now to 1-2 fish a tank per day but...
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Dear All, I wish to perform WISH on an adult zebrafish sample which is treated with collagenase and later acid-fixed (Formaldehyde + Acetic Acid). But before the WISH procedure, I would like to...
14 June 2017 6,072 1 View
Dear Researchers, I want to perform a whole mount ISH experiment on the zebrafish ovary. There are many papers in which they have done this experiment but the details are kind of missing. What I...
09 June 2016 6,431 3 View
Dear All, I have been trying to knock-in GFP in a particular locus at its N-terminus. I have prepared the Donor construct with a short arm of 0.8 kb and a long arm of 1.6 kb. I have been doing...
24 November 2015 9,032 4 View
Hi all, I am trying to make a construct by Gibson Cloning (NEB Kit). I have assembled 4 fragments and the total size is 6.5 kb. These fragments contain some intronic sequences from zebrafish and...
20 June 2015 3,678 3 View
