Ceren Günes

3 Questions 14 Answers 0 Followers

Questions related from Ceren Günes

Would longer ROCKi exposure change iPSC growth metabolism?

I am routinely using the ROCK inhibitor for iPSC passaging. 1 day after seeding, I normally switch to iPSC media without the ROCKi but once I left it over the weekend with ROCKi and surpassingly...

09 January 2020 1,936 4 View

Fluorescent signal reduction after fixation/permeabilization?

I infected human neural stem cells with a RFP tagged viral construct, and wanted to sort infected and undifferentiated cells. For this I use two intracellular markers, Sox2 and Nestin. When I ran...

21 January 2016 8,054 6 View

How long does it take a transduced vector to be expressed?

It depends on the cell type and also the viral construct, but is there an average timing for the viral vector to start its expression? I transduce human iPSC with shRNA lentiviral constructs, any...

03 December 2015 7,534 3 View