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Questions related from Ceren Günes
I am routinely using the ROCK inhibitor for iPSC passaging. 1 day after seeding, I normally switch to iPSC media without the ROCKi but once I left it over the weekend with ROCKi and surpassingly...
09 January 2020 2,005 4 View
I knockdown a gene by shRNA (lentiviral) in human iPSC. The shRNA construct is GFP tagged. What I observed under the microscope was, the GFP+ cells (the ones with shRNA, hence that gene of...
19 January 2017 3,897 4 View
I derived human NSPC from iPSC, and have been characterizing them. So far they differentiated into neurons and astrocytes easily in two weeks, and I decided to keep them longer (uninduced) for...
26 May 2016 7,274 6 View
I infected human neural stem cells with a RFP tagged viral construct, and wanted to sort infected and undifferentiated cells. For this I use two intracellular markers, Sox2 and Nestin. When I ran...
21 January 2016 8,209 6 View
It depends on the cell type and also the viral construct, but is there an average timing for the viral vector to start its expression? I transduce human iPSC with shRNA lentiviral constructs, any...
03 December 2015 7,612 3 View
Hi, I want to infect my CD34+ derived human iPSC with shRNA library, and to determine at which MOI values to test, I did some search but could not find much information. Has anyone transduced...
03 June 2015 8,097 5 View
I will reprogram human fibroblasts and CD34+ HSC with Sendai virus (CytoTune®-iPS 2.0). In the protocol they recommend coating with the plates with vitronectin, but as far as I know vitronectin is...
16 January 2015 4,942 9 View
I will do RNAi screen on hNSC with shRNA library, which has puromycin resistance cassette. Before starting that, I wanted to test at which concentration I could use puro on NSC to eliminate the...
06 November 2014 4,783 4 View
