Ceren Günes

5 Questions 24 Answers 0 Followers

Questions related from Ceren Günes

Would longer ROCKi exposure change iPSC growth metabolism?

I am routinely using the ROCK inhibitor for iPSC passaging. 1 day after seeding, I normally switch to iPSC media without the ROCKi but once I left it over the weekend with ROCKi and surpassingly...

09 January 2020 1,949 4 View

Fluorescent signal reduction after fixation/permeabilization?

I infected human neural stem cells with a RFP tagged viral construct, and wanted to sort infected and undifferentiated cells. For this I use two intracellular markers, Sox2 and Nestin. When I ran...

21 January 2016 8,122 6 View

How long does it take a transduced vector to be expressed?

It depends on the cell type and also the viral construct, but is there an average timing for the viral vector to start its expression? I transduce human iPSC with shRNA lentiviral constructs, any...

03 December 2015 7,549 3 View

Any advice on transduction efficiency in human iPSC?

Hi, I want to infect my CD34+ derived human iPSC with shRNA library, and to determine at which MOI values to test, I did some search but could not find much information. Has anyone transduced...

03 June 2015 8,037 5 View

Has anyone used Matrigel instead of Vitronectin for reprogramming with Sendai virus?

I will reprogram human fibroblasts and CD34+ HSC with Sendai virus (CytoTune®-iPS 2.0). In the protocol they recommend coating with the plates with vitronectin, but as far as I know vitronectin is...

16 January 2015 4,882 9 View