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Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...
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Hey everyone, For those that perform "homemade" ELISA, I have been titrating the different components of a sandwich ELISA. The most "acceptable" curve that I have obtained reaches an absorbance...
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Dear colleagues, I am trying to quantify astrocytes, neurons, and microglia in cortex and hippocampus. I would like to know how big should be the stack, for example, 20um??, the ideal objective...
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Hy everyone, Someone of you knows a good alternative for Iba1 rabbit antibody from WAKO company. The company has had problems with the production of the antibody and they are going to deliver it...
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