Carole-Keza Capitaine

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Questions related from Carole-Keza Capitaine

How do you grow LbetaT2 cells? Do you need specific DMEM, PBS/trypsin?

I have attempted to crack my LbetaT2 cells twice but both attempts have failed. The second time, I know that I might have exposed the cells to trypsin for too long. However, I think there might be...

10 November 2020 9,350 0 View

What could be the reason for 28S, 18S and 5S RNA band not moving from the wells? Could it be bound protein to the RNA?

I am extracting RNA from frozen human PBMCs using the Trizol Reagent method.

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