Carlos Galicia

2 Questions 3 Answers 0 Followers

Questions related from Carlos Galicia

Does transposase mRNA for Tol2 transgenesis in zebrafish needs to be polyadenilated?

Hello, I have experience producing transgenic zebrafish with CRISPR and the CAS9 mRNA would be polyadenilated with a Poly(A) tailing kit after in vitro transcription. However, with Tol2, the...

08 August 2016 336 2 View

How likely is it to wash off the target sample during the washing step of immunocapture PCR?

I have been doing immunocapture PCR but when I take a look at the gels they don't have PCR products. My controls indicate that the PCR worked so I was considering that maybe my sample is being...

05 May 2014 6,178 1 View