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Questions related from Carlos Moreno
I'm having a difficult time with harvesting mouse splenocytes. The protocol I'm using is as follows: Harvest spleen from mouse and place into 5mL of PBS Place a 70μm filter onto a 50mL conical...
23 June 2025 3,952 2 View
I'm trying to think through the best way to stimulate mouse CAR T cells that target mouse CD19 (1D3 scFv) without incubating the CAR T cells with B cells. I want to do this because a metabolic...
07 February 2025 5,540 1 View
I'm stimulating CD4+ T cells for 3 and 5 days with plate-bound CD3 antibody, soluble CD28 antibody, and LPS. I want to measure intracellular cytokine levels on days 3 and 5 (IL4, IFNγ, IL10, and...
15 August 2024 881 4 View
I'm working with Platinum-E (Plat-E) cells and generating viral particles that carry an insert of interest with a GFP reporter that will be used to infect mouse T cells. I understand that when...
22 June 2023 8,081 1 View
Hello all, I could really use some advice with T cell intracellular cytokine staining. I've been trying to stimulate CD4+ T cells isolated from mouse spleens and stain intracellularly for...
06 December 2022 5,838 3 View
I'm trying to determine what is the best way to activate my T cells using anti-CD3 and anti-CD28 antibodies. There are a few other topics that sort of address this question, but I still have some...
17 October 2022 7,038 5 View
I recently performed RNA sequencing on wildtype and knockout mice and have identified several differentially expressed genes (over 1,400 DEGs), and I'm interested in identifying what transcription...
22 November 2021 914 6 View
I'm culturing mouse mucosal and gingival epithelial cells and noticed these cell aggregates. Does anybody know what they may be? Is this the early stages of a fungal contamination? I thawed and...
17 June 2021 3,073 3 View
