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Questions related from Carlos Moreno
I'm working with Platinum-E (Plat-E) cells and generating viral particles that carry an insert of interest with a GFP reporter that will be used to infect mouse T cells. I understand that when...
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Hello all, I could really use some advice with T cell intracellular cytokine staining. I've been trying to stimulate CD4+ T cells isolated from mouse spleens and stain intracellularly for...
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I'm trying to determine what is the best way to activate my T cells using anti-CD3 and anti-CD28 antibodies. There are a few other topics that sort of address this question, but I still have some...
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I recently performed RNA sequencing on wildtype and knockout mice and have identified several differentially expressed genes (over 1,400 DEGs), and I'm interested in identifying what transcription...
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I'm culturing mouse mucosal and gingival epithelial cells and noticed these cell aggregates. Does anybody know what they may be? Is this the early stages of a fungal contamination? I thawed and...
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