Camilo Villouta

3 Questions 2 Answers 0 Followers

Questions related from Camilo Villouta

What is the best embedding medium (paraffin vs. resin) for fine spinach roots, and how do you locate roots in blocks after embedding?

Dear colleagues, I am currently working on studying the anatomy of spinach roots, particularly focusing on the fine roots and the main tissues. However, I’ve encountered some challenges in...

17 November 2024 2,691 2 View

How to normalize images acquired with different gain and different laser intensity?

Hi, my intern and I were working on imaging V. corymbosum buds in a confocal microscope (LSM900). Our plan was to have the same settings for all the images, but we changed (by mistake) the gain...

31 July 2023 9,056 2 View

How to quantify relative amounts of a substance with immunostainings through confocal microscopy when samples have high autofluorescence?

I am working with plant tissues, buds specifically, fixed in glutaraldehyde and embedded in LR White resin. I am working with the primary antibodies LM19 and LM20 targeting pectins. I am using a...

22 September 2020 5,423 6 View