Camilo Villouta

2 Questions 2 Answers 0 Followers

Questions related from Camilo Villouta

How to normalize images acquired with different gain and different laser intensity?

Hi, my intern and I were working on imaging V. corymbosum buds in a confocal microscope (LSM900). Our plan was to have the same settings for all the images, but we changed (by mistake) the gain...

31 July 2023 9,046 2 View

How to quantify relative amounts of a substance with immunostainings through confocal microscopy when samples have high autofluorescence?

I am working with plant tissues, buds specifically, fixed in glutaraldehyde and embedded in LR White resin. I am working with the primary antibodies LM19 and LM20 targeting pectins. I am using a...

22 September 2020 5,419 6 View