Camila F Almeida

2 Questions 2 Answers 0 Followers

Questions related from Camila F Almeida

Should my GOI and endogenous gene match about cDNA dilution factor and primer concentration?

I`m wondering about cDNA dilution and primers concentration of target and housekeeping genes in qPCR (using Sybr green) assay. Let`s suppose the following: by testing GOI primer efficiency (in a...

21 February 2016 6,818 2 View

Why do my C2C12 cells not differentiate only with 2% horse serum?

I'm trying to differentiate C2C12 cells using various media. I've tried 1% and 2% of horse serum and also insulin. The cells that received only HS did not differentiate (many died after 24h, the...

01 October 2015 2,363 2 View