Cahya Prihatna

3 Questions 7 Answers 0 Followers

Questions related from Cahya Prihatna

Removing RNA in bacterial genome for PacBio De Novo sequencing?

Hi, I need to sequence, whole genome, of a bacterium (Burkholderia species) using PacBio. However, it turns out (based on what the supplier says) that PacBio is very sensitive to RNA contamination...

14 September 2020 3,539 5 View

I'm having problems cloning a 3.2kb fragment. Does anyone have experience in single-step BP/LR gateway cloning?

Hello, does anyone have experience with single-step BP-LR Gateway cloning? I'm having problem to clone a 3.2 kb fragment. I did the same gateway cloning for an 800 bp fragment and I just saw this...

04 February 2016 3,086 4 View

Can anyone help me with low efficiency and aberrant tomato transformation using hygromycin and basta selection?

I've been struggling to get transgenic tomato using two different selections: basta and hygromycin. I did test to check the minimum concentration of both and got 10 mg/l for hygromycin and 0.5...

21 August 2014 9,832 8 View