Bushra Javaid

5 Questions 18 Answers 0 Followers

Questions related from Bushra Javaid

Primer designing from reverse translation of protein sequence

Hi, I am interested to design primers for my protein of interest (3' RACE). I have N-terminal amino acid sequence of protein. When I reverse translate that protein, codon usage table is required...

04 July 2017 9,203 3 View

How could I avoid galactomanan (gum) contamination during protein extraction?

I am using seed flour as starting material and Tris buffer for extraction.

28 February 2016 9,243 1 View

Hi, I need help to defat samples before protein extraction. Should I treat seed samples with hexane only or fresh samples also need treatment?

Hi, I am going to freeze dry samples prior to protein extraction for transport to some other destination. As seeds contain so much oil these are treated to defat them before protein extraction.I...

23 April 2015 5,362 5 View

How can one concentrate proteins after extraction from plant material?

I am extracting soluble proteins from plant material as well as seeds in bulk, after extraction I need to concentrate them. Do I have any option other than freeze drying?

28 January 2015 4,614 6 View

Nested pcr

Hi, I am doing nested pcr. I have completed round of pcr with outer primers now i am going for nested round of primers should i use pcr reaction as such or dilution of pcr reaction with outer...

17 October 2010 4,900 3 View