Burak Demir

2 Questions 3 Answers 0 Followers

Questions related from Burak Demir

Would using the same homoloyg arms with another cut site be possible?

I have a donor template with left and right homology arms (each 1000bp long) designed for a specific sgRNA cutting site but now I want to use another sgRNA cutting 15 bp downstream of the original...

11 July 2023 325 3 View

qRT-PCR melting curves are good for cDNA of overexpression cells but not for cDNA of parental cells. What could be the reason?

I am trying to find a good qRT-PCR primer set for a gene that has low expression in our cell lines. As you see in the attachment I have a very sharp one peak when I used cDNA from overexpression...

24 February 2020 2,604 5 View