Büşra Yirmibeş

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Questions related from Büşra Yirmibeş

Is there any specific condition or protocol adjustment required for efficient amplification of attB-containing primers with Q5?

I am working on Gateway cloning and using primers that contain attB sites. The primers are 49–50 nucleotides long, and their melting temperatures (Tm) are approximately between 70–76 °C. However,...

18 July 2025 5,582 1 View

How can I design my reverse primer, if destination vector has a tag like GFP in C terminus in Gateway cloning?

Hello everyone, I designed primers for Gateway cloning, but I'm unsure about the sequences I need to add. I plan to transfer my genes along with their native promoters into a donor vector. I added...

20 January 2025 3,147 2 View

How can I find detailed promotor sequence of a gene?

I will be doing Gateway cloning and need to use the native promoter. I found some databases for that, like EPD, but I couldn't find any information for three of the genes. Are there any other...

21 September 2024 3,815 3 View

How can I prepare my shutdown solution for flow cytometry?

Is there a formula for preparing of shutdown solution for flow cytometry. I found a formula as water (%99,79), 2-phenoxethanol (0,20%) and sodium benzoate (0,01%). But I am not sure that because...

02 January 2024 5,967 3 View