Bryan Clifton

5 Questions 7 Answers 0 Followers

Questions related from Bryan Clifton

Does it matter if antibodies are monoclonal or polyclonal for Quantitative Western Blots?

Hello helpful people, I am planning a quantitative western blot experiment and have the choice between a polyclonal and monoclonal antibody. I have never done western blots personally. Does...

12 November 2020 987 4 View

Is it possible to use CRISPR to swap stop codons with GFP or other tags in Drosophila?

I want to visualize a protein’s localization and also identify the components of the complexes it forms at its endogenous expression levels in Drosophila. I would like to just swap out the stop...

07 May 2016 3,947 3 View

Are there specific N-terminus protein domains that one can use to differentiate the cytoplasmic and axonemal dynein intermediate chains?

Are there domains that say this intermediate chain protein is for sure a part of an axonemal or cytoplasmic dynein complex? Does the IC have to have the N-terminus coiled-coil and serine-rich...

02 November 2015 2,191 1 View

Can I use TB, LB, or SOC in place of NZY+ broth for transformations using SURE 2 Supercompetent cells?

I am doing a cloning experiment where my vector has the gateway cassette so I need to use SURE 2 cells as they are ccdB immune. However, the protocol for SURE 2 calls for NZY+ broth but our lab...

18 August 2015 2,207 3 View

Does anyone know of any good references genes for comparing ovaries and testes in Drosophila melanogaster via qRT-PCR?

I am trying to do qPCR to compare the expression of genes between adult virgin ovaries and testes, however, I have been testing many reference genes and so far I have only found one gene (clot)...

14 November 2014 4,934 1 View