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Questions related from Bruna Bittencourt
Hi everyone! I am trying to detect ATM expression in Hacat cells through western-blot. ATM is a large protein (350kDa) and I have tried running 60ug of lysate - 6% polyacrylamide gel for 3.5h...
06 June 2019 6,736 2 View
Hello! Could anybody suggest a transfection concentration for mir181-a? I have seen in papers people using around 50nM for transfection of mir181-a mimic, however I feel that such a high...
05 May 2019 9,524 6 View
Hello! I am doing a microRNA over expression experiment. One of it is targets is ATM, which is down regulated upon correct transfection/ over expression of the microRNA of interest. Can I detect...
05 May 2019 7,416 4 View
I have a transfection experiment to be done. The stock miRNA is 5nMol. The manufacturer recommends adding 75uL of sterile water, giving me a concentration of 66.67 uM. How can I have a final...
05 May 2019 325 8 View
I have to extract genomic DNA from skin cells collected on a swab. I am currently using the PureLink Genomic DNA Kit, and following the protocol for the Mammalian Cells Lysate, however they...
04 April 2019 5,988 4 View
What can I use as an apoptosis inducer in ID8 cell lines that will not interfere with MTT or WST-1 proliferation assays? (all colorimetric assays) Has anybody used heat? or ethanol?
07 July 2017 3,276 5 View
Have anyone used the WST-1 assay for ID8 cells? Is 1000 cells a good starting amount to seed them? Can I induce cell death to my cells using Triton X and then use the WST-1 assay after to measure...
07 July 2017 7,104 4 View
