Brinley Harrington

3 Questions 2 Answers 0 Followers

Questions related from Brinley Harrington

Results Differ from ELISA with Multiplex, Same Samples?

Hi I recently ran a multiplex assay examining GM-CSF, IL-6, IL-8, IL-10, IL-1β, and TNF-α. After analyzing the data, I saw different results for my IL-6 and IL-8 than when I previously ran these...

07 June 2025 2,570 1 View

Detection of IL-10 in Media samples using HPLC?

Hello I am trying to develop a quick way of seeing the concentration/presence IL-10 in culture media samples. I have used ELISA, but it is time-consuming and not every good for a couple of...

30 October 2024 172 3 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

31 July 2024 7,112 1 View