Brian Andrew Gill

3 Questions 2 Answers 0 Followers

Questions related from Brian Andrew Gill

Can you help with my gel electrophoresis?

Hey everyone. I'm having gel electrophoresis problems all of a sudden and haven't been able to determine why. The photo is a 1% TBE gel pre-stained with 10 ul gel green run at 95V for an hour....

04 April 2018 5,213 4 View

DNA Metabarcoding (COI) with Fusion Primers; Which taq DNA polymerase?

Hey Everyone. I am trying to DNA metabarcode (COI) bulk samples of aquatic insects.I would like to use fusion primers consisting of an Illumina MiSeq flow cell bind, sequencing primer binding...

08 August 2016 9,818 3 View

What is the proper way to extract DNA for DNA metabarcoding? Subsample tissue or subsample pooled extraction products?

Dear colleagues. I am extracting DNA from bulk samples of stream insects for DNA metabarcoding. I am using a TissueLyser to disrupt and homogenize the sample and then using a Qiagen kit for...

01 January 2016 214 4 View