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Hi all, I'm trying to image some tissue that has Tdtomato onboard, and would like to quench/kill these. I can't use heat antigen retrieval to kill these fluors, and I've explored UV light exposure...
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It is my understanding that you don't add a polyA in lentiviral vectors, as it is contained within the LTR. However, in some circumstances, there is no obvious polyA in simple plasmids which are...
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I am doing practice runs of an ES cell lysis protocol that I am planning on using for southern blot analysis. I get the standard spidery precipitated DNA after addition of isopropanol, I spin it...
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Short question: Suggestions for a purification method for a 20 kb plasmid that will give near-quantitative yield of the original input that is low salt? --- Long explanation: I'm interested in a...
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