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Questions related from Brian Lin
Hi all, I'm trying to image some tissue that has Tdtomato onboard, and would like to quench/kill these. I can't use heat antigen retrieval to kill these fluors, and I've explored UV light exposure...
28 April 2021 9,333 4 View
It is my understanding that you don't add a polyA in lentiviral vectors, as it is contained within the LTR. However, in some circumstances, there is no obvious polyA in simple plasmids which are...
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I am doing practice runs of an ES cell lysis protocol that I am planning on using for southern blot analysis. I get the standard spidery precipitated DNA after addition of isopropanol, I spin it...
07 November 2013 5,668 5 View
Short question: Suggestions for a purification method for a 20 kb plasmid that will give near-quantitative yield of the original input that is low salt? --- Long explanation: I'm interested in a...
02 April 2013 2,109 12 View
Where do people buy their dyes for conjugation: I'm looking for basically the cheapest place for alexa fluors or equivalent which is still of usable quality. We're based in the US, for...
30 May 2012 9,734 5 View
