4 Questions 35 Answers 0 Followers
Questions related from Brian Lin
Hi all, I'm trying to image some tissue that has Tdtomato onboard, and would like to quench/kill these. I can't use heat antigen retrieval to kill these fluors, and I've explored UV light exposure...
28 April 2021 9,281 4 View
It is my understanding that you don't add a polyA in lentiviral vectors, as it is contained within the LTR. However, in some circumstances, there is no obvious polyA in simple plasmids which are...
04 February 2014 6,610 12 View
I am doing practice runs of an ES cell lysis protocol that I am planning on using for southern blot analysis. I get the standard spidery precipitated DNA after addition of isopropanol, I spin it...
07 November 2013 5,627 5 View
Short question: Suggestions for a purification method for a 20 kb plasmid that will give near-quantitative yield of the original input that is low salt? --- Long explanation: I'm interested in a...
02 April 2013 2,063 12 View
