Brendan Mormile

2 Questions 2 Answers 0 Followers

Questions related from Brendan Mormile

What is your indel percentage during CRISPR protoplast transfection?

I am performing CRISPR transfections in citrus protoplast using RNPs. I have seen about 0-29% indel percentages 24-48 hours post transfection using 40% PEG and different sgRNAs. I screen for edits...

13 May 2025 3,359 0 View

Why isn't GUS expressing in transgenic plants?

I have been conducting gene expression assays using a GUS reporter in transgenic Arabidopsis plants. Expression was strong when I applied my positive controls, however when the seed stock started...

05 September 2018 7,392 7 View