Braeden Giles

2 Questions 2 Answers 0 Followers

Questions related from Braeden Giles

Looking for positive control for mTOR in western blot

Hello, I'm currently trying to run an experiment in which i'm looking to see mTOR protein expression across a pre-treated cancer line arising from epithelial cells. I was considering using...

01 January 1970 2,189 1 View

Clustering Issues with THP-1 Macrophages

I am currently culturing THP-1 macrophages using 75 ng/ml PMA over the course of 120 hours. I have noticed that while the cells are attached to the well... I lose substantial cell numbers if the...

01 January 1970 6,995 4 View