Braeden Giles

3 Questions 2 Answers 0 Followers

Questions related from Braeden Giles

Why does BODIPY fluorescence increase in Atorvastatin treated macrophages?

Hello, We are examining a toxicant that increases the number of lipid droplets in THP-1 macrophages. We think this is due to agonism of cholesterol biosynthesis and subsequent storage in the...

03 April 2025 2,997 2 View

Looking for positive control for mTOR in western blot

Hello, I'm currently trying to run an experiment in which i'm looking to see mTOR protein expression across a pre-treated cancer line arising from epithelial cells. I was considering using...

01 January 1970 2,231 1 View

Clustering Issues with THP-1 Macrophages

I am currently culturing THP-1 macrophages using 75 ng/ml PMA over the course of 120 hours. I have noticed that while the cells are attached to the well... I lose substantial cell numbers if the...

01 January 1970 7,039 4 View