Bradley James Stevenson

2 Questions 10 Answers 0 Followers

Questions related from Bradley James Stevenson

Using sodium ion adducts in LC/MS for quantification of steroids?

I am interested in quantifying a range of steroids by LC/MS (C18 column with 70% methanol and 30% 25 mM ammonium acetate mobile phase, using atmospheric pressure electrospray ionisation) and...

07 July 2013 6,870 26 View

Enzyme leakage from E. coli cultures or colonies?

I have been expressing a cytoplasmic sulfatase (from Pseudomonas aeruginosa) in E. coli DH5-alpha and have found significant enzyme activity in the culture media (either LB or M9 minimal media...

01 January 2013 6,543 15 View