Borrero C

6 Questions 15 Answers 0 Followers

Questions related from Borrero C

Which are the differences between PEG8000 and TCA precipitation methods?

Is there a limit on the protein size? Which parameters should I have in mind to choose one or the other?

09 September 2013 8,735 0 View

Can someone answer a question regarding TMB substrate - ELISA?

I did a little test to see if my substrate and conjugated Ig were ok. When I added 1ul of anti Ig-Y (secondary conjugated antibody) directly to 1ml of my TMB working solution it turned orange...

07 July 2013 3,474 5 View

Can someone suggest an online software suitable for the primers design?

Is there any online site where you can put the sequence and search if it has sites where you can use universal primers (T7 or M13)?

07 July 2013 1,084 9 View

How to quantify recombinant protein produced?

How to quantify recombinant expressed protein if some is secreted to medium and some gets attached to the membrane? (also some inside the bacteria but I'm not interested in this fraction). I...

07 July 2013 9,409 9 View

Replacement of SDS

Which other reagent can I use ?

06 June 2013 7,243 7 View

Could someone help me solve some troubles with my competitive ELISA? Obj: Detect chicken serum IgY. No color development even using a positive control

I´m using as secondary antibody: HRP Conjugated anti chicken IgY (http://www.genetex.com/Chicken-IgY-HL-antibody-HRP-GTX26753.html) and TMB substrate...

06 June 2013 686 10 View