Bornali G

3 Questions 7 Answers 0 Followers

Questions related from Bornali G

How can we elute a protein band of interest?

I would like to elute a protein band for a 10 % SDS-PAGE gel. The amount of protein loaded is from a 5ml induced culture, pelleted and mixed with lamelli buffer. I would like to use the protein...

06 June 2015 8,608 5 View

Can anyone suggest how to remove imidazole from the purified protein samples?

There are two protein which i am trying to purify for my in-vitro assays. 1. A his tagged protein which i have eluted in 50 mM NaH2PO4, 50 mM NaCl and 250 mM imidazole. Now what buffer...

12 December 2014 6,974 10 View

Can anyone suggest how to prepare SYBR green mix in the lab for real time PCR experiments?

I would like to learn how to prepare SYBR green master mixes for real time PCR in the lab?

11 November 2014 3,038 5 View