Boniface Kimathi

5 Questions 7 Answers 0 Followers

Questions related from Boniface Kimathi

How is a t-test on fold change calculated?

How does one determine whether a fold change calculated on qPCR data using 2-ΔΔCt method is significant? Answers provided to a similar question earlier indicate that one should do a one sided...

10 October 2014 6,004 2 View

How do I avoid formation of polymers of my protein of interest in Western Blot?

I have done western blots to detect a 19kDa protein. I am getting signals representing polymerization of the protein.This would not have been a problem but there is very little or no signal where...

03 March 2014 1,849 5 View

Can someone suggest how to get a consensus sequence for a gene?

I am trying to get the consensus sequence from the same genes so that i can use it to design primers. I have aligned them in ClustalO and can see the sequence in JalView. How do I export it to a...

07 July 2013 4,245 2 View

What are the best kits to carry out glycosilation and acetylation assays on proteins?

What are the best kits to carry out each of the following assays on proteins for comparison between two groups of samples? 1. Gycosilation. 2. Acetylation. 3. Editing. I am looking for possible...

03 March 2013 5,705 5 View

Where can I get chicken apolipoprotein A1 antibody for Western Blot?

I tried Abcam, but they do not have that. Any suggestions?

01 January 2013 3,570 2 View