Bogumila Swietek

4 Questions 5 Answers 0 Followers

Questions related from Bogumila Swietek

How long can calcium dyes like fura-2 and flu-4 stay inside cortical neurons?

Hello All, I am trying to do calcium imaging in primary cortical neurons at long-term time points like 6 hrs after imposing treatment. Does anyone know how fast can calcium dyes stay in neurons...

11 November 2018 4,867 2 View

Does anyone know of a company that can manufacture custom cell culture inserts (transwell)?

Hi, Does anyone know of a company that can manufacture custom cell-culture inserts or transwells?

10 October 2018 684 0 View

Can you detect TNF alpha in LPS stimulated astrocytes using western blot ?

Hello. Can you detect levels (pg/ml) of TNFa and IL-1B in LPS stimulated primary rat astrocytes by using a western blot? If yes, what is the optimal concentration of LPS and how long should the...

04 April 2018 8,678 4 View

How can I detect poly-l-lysine adherence to a substrate?

I want to detect (using fluorescence) whether my poly-l-lysine is uniformly adhering to a substrate. Anyone have any suggestions for a dye or chemical which I can use? Thank you in advance.

02 February 2018 7,408 3 View