9 Questions 14 Answers 0 Followers
Questions related from Blerta Stringa
Hi everyone, have you aver used K-PIPES buffer? how to prepare it and in case, is there any other commercial buffer that I can use for fixation?
20 April 2022 4,815 2 View
Hi, I am interested to know the DNA sequence of the TF corrisponding to the DNA binding domain. Do you know any tool developed recently that can help?
15 November 2020 9,758 6 View
How is it possible that RT-qPCR demonstrate a down-regulation of my interested gene (from 22 to 27 ct) while WB is showing the protein expressed with the same intensity as in cells where the gene...
27 September 2020 2,956 15 View
Hi everyone, I am trying to set up an experiment with the aim to identify putative transcription factors recruited in a functional regulatory region located in a non coding portion of human...
13 June 2019 7,605 2 View
Hi everyone, I am trying to do some experiments with HS578BST cell line (normal breast cell line) but I have big problems on growing them. I am using DMEM (with 10% FBS) and EGFR supplied every...
17 November 2018 9,286 3 View
Hi I am trying to electroporate a phosphorilate PCR product in my cell line. As the amount is very low, I was wondering if I can skip a purification step after phosphorilation. Does the T4 DNA...
18 October 2017 1,911 2 View
I would like to prepare by my self a gradient gel to separate proteines. I did some research but I don't have the right equipment to do it, such as cylindres or the chamber system adequate for...
25 March 2015 6,871 7 View
Hi all, I am trying to extract RNA from 12 well plate and so far the yield obtained from trizol/chloroform protocol is OK. After that I tried to do DNAase step (using NEB DNAse I) but I found...
01 January 1970 2,791 3 View
Hi all, have you ever realized how many protocols are available for mesoderm differentiation??? Example: CHIR+ACTIVIN in E6 medium, CHIR+MIM BMP+Ascorbic Acid, RPMI+Ascorbic Acid... What...
01 January 1970 2,835 1 View
