Biswaranjan Paital

9 Questions 36 Answers 0 Followers

Questions related from Biswaranjan Paital

Can soft tissues be transported in glycerin or in any materials (to minimize degradation) for direct imaging after fluorophore exposure?

If certain fluorescence experiments are done in field and the soft and degradable tissue materials (brain, liver etc.) need to be transported to laboratory (24 hr travel time), can the material be...

09 December 2018 1,049 0 View

Why an enzyme shows increased activity with respect to time (h) with unlimited substrate in a controlled system?

During standardization of an enzyme to study its activity, it showed a linear trend of increase up to 4 h then it gets stabilized. For the first time I got such an enzyme to have its maximum...

11 August 2014 6,888 9 View

PCR for Annexin II in cancer cell lines - can anyone help?

I used the primers (5′- GATGTGAAACACTTTGCTCCT-3′ (sense) and 5′-ATAGCGACACTTGGATAGGGG-3′ (antisense) cited in different literature's for Annx II to have RT-PCR amplification in cancer cell lines...

20 September 2013 5,322 3 View

Why redox stress is a stress for cells?

One of the modern techniques of analyzing oxidative stress is to measure redox status of cells. Many times redox status is also referred as redox stress. By nature all molecules tend to be in...

13 September 2013 5,854 1 View

Can autoclaved water be used for buffer preparation?

To get rid of microbial and related contaminations, buffers are autclaved after preparation. However, some buffers are not high temperature resistant such as MOPS, HEPES etc. Therefore, autoclaved...

30 August 2013 6,723 8 View

During very primitive evolution why was oxygen but not nitrogen used by the cells for example cyanobacteria and others.

When life arose in earth oxygen was sensed by the first living cells as a source of helper gas for energy production. May be it is due to the subsequent development or already developed...

21 August 2013 3,262 6 View

Can anyone explain the reason for the existence of extra-cellular superoxide dismutase enzyme but not extra-cellular catalase?

Most of the ROS metabolism occurs in the mitochondria or cytoplasm. What could be the major source of superoxide radicals at extra-cellular places, causing the tissue to develop extra-cellular...

28 March 2013 9,053 16 View

Is there any method to measure thickness of the membrane of the amniotic sac or fetal membrane in a pregnant woman?

A fetus is developed within the amniotic sac. Is there any method to measure the thickness of the membrane of the amniotic sac?

22 February 2013 2,745 8 View

No RNA bands during plasmid isolation

We have been following the conventional phenol chloroform method for plasmid isolation. At the same time we are also keen about the RNA bands on AGE. For this, we have omitted RNAse treatment at...

01 January 1970 1,253 6 View