Biswaranjan Paital

3 Questions 19 Answers 0 Followers

Questions related from Biswaranjan Paital

Why an enzyme shows increased activity with respect to time (h) with unlimited substrate in a controlled system?

During standardization of an enzyme to study its activity, it showed a linear trend of increase up to 4 h then it gets stabilized. For the first time I got such an enzyme to have its maximum...

11 August 2014 6,845 9 View

PCR for Annexin II in cancer cell lines - can anyone help?

I used the primers (5′- GATGTGAAACACTTTGCTCCT-3′ (sense) and 5′-ATAGCGACACTTGGATAGGGG-3′ (antisense) cited in different literature's for Annx II to have RT-PCR amplification in cancer cell lines...

20 September 2013 5,266 3 View

No RNA bands during plasmid isolation

We have been following the conventional phenol chloroform method for plasmid isolation. At the same time we are also keen about the RNA bands on AGE. For this, we have omitted RNAse treatment at...

01 January 1970 1,200 6 View