Birendra Kumar Singh

8 Questions 5 Answers 0 Followers

Questions related from Birendra Kumar Singh

What's the best way to stain whole blood with CD4 antibody conjugated with fluorochrome (PE, FITC)?

I am staining whole blood with CD4 FITC/CD4PE antibody. However, after trying so many buffer conditions I cannot able to see the fluorescence of FITC/PE. I am using a fluorescence microscope for...

06 April 2020 4,985 0 View

Could Anyone suggest the protocol for staining CD 4 and CD 8 in whole blood. Which buffer is suitable for the staining?

I am staining the CD4 and CD8 markers in the whole blood sample. However, when I used the PBS buffer (pH. 7.0 to 7.4) I have no results. Then I tried 2mmEDTA PBS buffer but still, there is no...

25 March 2020 422 3 View

B cell, T cell and NK Cell specific marker for Human. To calculate CD4% ratio and CD8?

Dear Scientist What is the specific marker for T cell, B cell and NK cell. So that i can calculate the CD4%, ratio and CD8 number for microscopy. I am currently using the CD4 FITC, CD8 PE and...

16 January 2018 6,345 0 View

PH 9 is good for WBC or not?

I am working with whole blood and conjugating the WBC with CD4 FITC. I am using the PBS buffer containing 2mM EDTA (pH 7.2). However, I m not getting the result. I found that CD4FITC works well...

12 January 2018 6,083 4 View

How to proceed for the counting of CD4, CD8 and CD19 from whole blood sample without isolating the WBC?

I am doing experiment with BD antibody conjugated with CD4 FITC, CD8 PE and CD19 perCp. However, I am not able to detect with higher concentration also. I need some information regarding the...

24 October 2017 8,194 2 View

Why the self-made buffers and solution crystalize after some time but not the commercial buffers and solutions?

When I make the TAE buffer and TBE buffer in the laboratory, then after keeping it for long time I observe that there are some crystals. However, when I purchase it commercially then there are no...

25 July 2017 4,470 2 View

How to count the live and dead cell and how to distinguish except the trypan blue technique. As, I know trypan blue only stains the dead cell?

I want to count the live and dead cell. So which is the best method and how to do that.

06 February 2017 2,688 3 View

What solvent (and concentration) is suitable to dissolve essential oil (water or DMSO)? Is there any complication for using it for cell test?

Actually I am extracting essential oil from plant sample using ethylacetate. But its very sticky and I am looking for the best solvent do dissolve the sticky sample.

01 October 2015 10,025 13 View