Bindu Subhadra

6 Questions 13 Answers 0 Followers

Questions related from Bindu Subhadra

Is it possible to conduct live cell imaging over a course of time using confocal microscopy in 3D culture?

Hi I am new to 3D culture, live cell imaging and confocal microscopy. Is it possible to visualize epithelial and endothelial cells by live cell imaging for a period of time using confocal? In...

03 May 2023 1,653 8 View

Program to find out the number of genes in internal clusters?

Hello friends I am analyzing the whole genome sequence of bacterial origin. I would like to know whether there is any user-friendly software to find out the number of genes in internal clusters...

27 February 2017 8,307 3 View

Difficulty in whole genome analysis?

Hello all, I am doing whole genome analysis of an E. coli strain and finding it difficult to identify the followings. 1. Pseudogenes2. Genes with function prediction3. Genes with pfam domains4....

21 June 2016 3,774 7 View

Mummer plot analysis?

Hi, I am new to comparative genome analysis. I have created a Mummer plot using the whole genome sequence of my strain of interest and a control strain. However, I face difficulty to read the...

31 May 2016 6,793 3 View

Trouble making phylogenetic tree with more than one gene?

Hi all, I am having trouble making the phylogenetic tree. I have sequenced a new strain of Escherichia coli and used the clustal omega to make the tree using individual house keeping genes....

18 May 2016 2,259 3 View

How to prevent dye from precipitating while making CAS agar plate for siderophore detection?

Hi, I am trying to detect siderophore production in Mycobacterium using CAS (Chrome Azural) agar method. Protocol for the preparation of blue dye is shown below. Blue Dye: Solution 1:Dissolve 0.12...

11 January 2016 1,875 4 View