Binaya Adhikari

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Questions related from Binaya Adhikari

Treating/'fumigating' herbarium specimen: is -80 C OK?

I am aware of the use of -20 C to -40 C range freezing for treating herbarium specimen. However, I am not sure if -80 deg. C would also work. Does anyone have any information/experience with it?...

27 February 2020 6,645 4 View

Could anyone recommend me seed germinating protocols for these species: 1. Lobelia cardinalis, 2. L. puberula, 3. L. spicata and 4. L. inflata?

Lobelia siphilitica needs 2 months of stratification in cold to break seed dormancy. Does any of the other species I mentioned have a similar or a different requirement?

28 August 2015 4,780 6 View

How might I best deal with heteroplasmic mitochindrial DNA sequences in population genetic analyses such as genetic diversity?

Some mitochondrial DNA samples consistently show sequence heteroplasmy indicating that both types of sequences occur within the population. People usually tend to discard heteroplasmic samples...

24 May 2015 7,551 4 View

Is there an upper length limit for a DNA probe for Northern?

Many people suggest that the DNA probe for Northern needs to be minimum of 300bp long. Is there a upper length limit? I am trying to troubleshoot Northern using plant RNA and many people are...

01 May 2015 6,568 3 View