Bina Prajapati

4 Questions 6 Answers 0 Followers

Questions related from Bina Prajapati

What might cause aggregates in Schneider's media supplemented with hygromycin?

Hi all, I am trying to establish stable drosophila embryonic line S2R+ and planning to use hygromycin (have pCoHygro plasmid for co-transfection)as a selection drug for the transfected cells. I...

02 February 2017 5,070 3 View

Anyone here with experience on western blot for whole fly lysate?

Hi, I am testing a commercial importin antibody generated in rabbit with drosophila fly lysates. And i get strong bands of wrong size (unspecific bindings) whereas i cant find any bands of correct...

08 August 2016 5,850 3 View

Are there any enrichment methods to grow S2R+ cells?

I am trying to culture S2R+ cells from frozen (liq. Nitrogen) aliquots. I am following the standard growth conditions as suggested on standard protocols, but have not been able to grow them...

07 July 2016 946 2 View

Anybody here who knows a cheaper plus efficient Geminin (rabbit)antibody for IF and WB?

I would like to shift to a cheaper anti-Geminin(rabbit) antibody for my western blot and IF staining. I have been using an expensive one from Abcam. If anybody here happens to be using the same...

02 February 2016 7,999 2 View