Bikal Ghimire

2 Questions 4 Answers 0 Followers

Questions related from Bikal Ghimire

Any suggestions for extraction of high concentration of plasmids, Phytophthora sp. ?

I have tried NucleoSpin® Plasmid and Qiagen Mini-Prep Kit and got just between 30-70 ng/ul DNA from 4 ml culture in both case. The plasmid I am trying to isolate is high copy. Do you think I...

02 February 2020 270 2 View

Purification of Cas9 and T7 polymerase?

I am trying to purify Cas9 and T7 for my experiment but I am not being able to get purified proteins. For pre-assy, I used PBS 1x buffer to wash pellets, lysozyme and DNAse I used, sonication for...

04 April 2019 7,185 5 View