Bhumika Kulai

2 Questions 3 Answers 0 Followers

Questions related from Bhumika Kulai

How to express GOI in pESc-His vector,with Gal promoter? Did lysis by NaOH+LiAc method, no protein band. How to proceed?

Currently trying to express protein in S.cerevisaie,peSc-His vector which has Gal promoter for induction.Did expression on enriched YPD/YPG media,suggested SD/SG media and addition of 1%galactose...

06 June 2018 3,248 0 View

I plan to express protein in Halobacterium salinarum. I require info on expression efficiency of H.salinarum as host and the protein yield?

Planning to express recombinant protein in H.salinarum. And need information on vectors of H.salinarum

10 October 2017 469 2 View